Serial dilutions of stock gblock DNA of Spirinchus thaleichthys
Today I am going to use the Agilent 2100 Bioanalyzer to assess the size and quality of DNA of the gblock DNA I ordered for the Spirinchus thaleichthys.
Yesterday I took the stock concentration of the gblock from IDT that was 1.84 x10^8 molecules/uL and diluted it 10x to 1 molecule/uL. Now, I want to assess the quality of the DNA through each of the dilution steps.
The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA and RNA. ... The fluorescent dye molecules intercalate into DNA or RNA strands. They are then detected by their fluorescence and translated into gel-like images (bands) and electropherograms (peaks).