• bkamermans8

Cytochrome b and qPCR

Updated: Feb 10, 2021

Cytochrome b is a protein in the mitochondria (see schematic below #9) of eukaryotic cells. The mitochondria is a double layered organelle found in eukaryotic cells (see TEM image below).

Schematic of an animal cell from Wikipedia (

TEM image of mitochondria of a mammal cell from Wikipedia (

Cytochrome b is a component of respiratory complex III. It is a protein that functions as part of the electron transport chain. The electron transport pumps protons to create a proton motive force. Proton gradient is used for the generation of adenosine triphospate (ATP). ATP is an organic compound that provides energy to eukaryotic cells.

Mitochondrial cytochrome b is among the most extensively sequenced genes to date across the vertebrates.

In fact, I am using previously published cytochrome b data from Longfin Smelt (Spirinchus thaleichthys) we designed primers and probes for qPCR. I used Geneious to align sequences downloaded from the sequence database, NCBI (National Center for Biotechnology Information).

Once I had a consensus sequence based on previously published cytochrome b genomic data, my mentor from the University of Alaska Fairbanks, Andres Lopez designed two qPCR assays for us to test out.

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